Equipment

带有TIRF的Nikon N-Storm超分辨率显微镜系统

这种新的尼康N态超分辨率系统是针对单分子成像量身定制的，使用随机光学重建显微镜（Storm）和/或光活化的定位显微镜（PARM）具有高精度定位，以创建具有20nm的空间分辨率（10倍）的图像（10倍）在传统的光学显微镜上），能够在2D和3D模式下进行多色成像。总内反射荧光（TIRF）显微镜功能。
主要功能包括：
6个激光线	目标
405 nm LD 20 mW 445 nm LD 20 MW 488 nm DPSS 70MW 514 nm DPSS 40MW 561 nm DPSS 70mW 647 nm LD 125 mW	CFI计划Apo Lambda 4X CFI PLAN APO LAMBDA 10X CFI计划Apo Lambda 20X CFI计划荧光40x油 CFI HP APO TIRF AC 100X油

Nikon N-SIM Structured Illumination Super-Resolution Microscope System

这system uses Structured Illumination technology, which gives a two-fold enhancement in resolution (over conventional optical microscopy). 具有在2D，3D和TIRF-SIM中实时样品中动态事件图像动态事件的能力（更高的信噪比，以进一步增强分辨率）。 Includes top of the line super-resolution and TIRF objectives (100X) have superior optics with the least amount of lens defects/aberrations. A wide-ranging illumination and detection systems to give users greater flexibility.
主要功能包括：
6个激光线	目标
405 nm LD 20 mW 445 nm LD 20 MW 488 nm DPSS 70MW 514 nm DPSS 20 MW 561 nm DPSS 70mW 647 nm LD 125 mW	CFI PLAN APO LAMBDA 10X CFI计划Apo Lambda 20X CFI HP APO TIRF AC 100X油

尼康A1R共聚焦激光显微镜系统

Nikon A1R是一个模块化共聚焦系统，由单独的扫描头组成，标准4 PMT检测器模块以及用于伪二进制成像，控制模块，激光模块和光谱检测器模块的额外PMT。A1R具有高达4096 x 4096像素的标准高分辨率共聚焦模式，以及一个高速谐振模式，能够在512 x 64像素下扫描230帧。除了基于激光的共聚焦图像采集外，该尼康平台还利用了高端数码摄像头进行非焦点图像采集。这updated A1R has a new multi-detector unit with 2 GaAsp PMT detectors to give brighter signals, enhanced signal-to-noise ratio, and less phototoxicity. 这updated A1R has the ability to perform Enhanced Resolution algorithms on point scanning confocal datasets to further increase resolution of images, and also Retirement/Replacement of Confocal Workstations allows for future expansion and faster processing, including GPU 3D Rendering.
主要功能包括：
6个激光线	目标
405 nm LD 20 mW 445 nm LD 20 MW 488 nm DPSS 70MW 514 nm DPSS 40 MW 561 nm DPSS 70mW 640 nm LD 125 MW	10X Plan-Fluor/0.3 NA w/Phase 20x Plan-apo/0.75 Na 40x PLAN-FLUOR/0.75 NA 40X Plan-Fluor/1.3 NA Oil 60X Apo TIRF/1.49 NA Oil 100X PLAN-APO/1.4 NA OIL

Nikon A1共聚焦激光显微镜系统 + picoquant

尼康A1是一个基本的共焦系统组成of a separate scan head, standard 5 PMT detectors, control module, and laser module. The A1 has a new multi-detector unit with 2 GaAsp PMT detectors to give brighter signals, enhanced signal-to-noise, and allow less phototoxicity. High-resolution confocal mode permits up to 4096 X 4096 pixels. This laser scanning confocal system is similar to that of the Nikon A1R in that it is configured on the Nikon TiE Widefield, inverted, fluorescence microscope. NIS Elements imaging software is used to control acquisition and analysis. 这picoquant成像系统是添加到Nikon A1系统中的升级程序包，以执行荧光寿命成像（FLIM）。该系统采用脉冲激光器来评估蛋白质 - 蛋白质相互作用，并且是IBP成像核心的独特之处。该系统配备了439 nm（121 MW峰值功率）和483 nm（136 MW峰值功率）激光器。 5 pmts（光电倍增管）自动对焦系统尼康领域宽场荧光显微镜
主要功能包括：
4个激光线	目标
100 mW时405 nm二极管 488 nm diode at 350 mW 480 MW的561 nm二极管 100 mW时的640 nm二极管	10倍平行/ 0.3 na w/ phase 20x Plan-apo/0.75 Na 40x PLAN-FLUOR/0.75 NA 40X Plan-Fluor/1.3 NA Oil 60X PApo TIRF/1.49 NA Oil 100X PLAN-APO/1.4 NA OIL

Lambert LIFA Fluorescence Lifetime Imaging Microscopy System

Lambert Flim系统是一个完全开发的系统，用于具有高精度，稳定性和获取/处理速度的荧光寿命的频域测量。
主要功能包括：
尼康领带广场荧光显微镜
高功率多领单元，峰值排放率：	目标
405nm (BFP) 447nm（CFP） 479nm (GFP) 532nm (RFP)	10X Plan-Fluor/0.45 NA w/phase 20x Plan-apo/0.75 Na 40x PLAN-FLUOR/1.30 NA OIL 60X PLAN-APO/1.40 NA OIL

钙钻机

这Nikon TiE Imaging System is a high resolution system designed for fluorescence imaging of small molecules in living cells. It is a filter-based, wide-field fluorescence system designed for measurement of intracellular signaling molecules including calcium ions, hydrogen ions (pH), nitric oxide, and other small molecules using appropriate fluorophores. Nikon’s NIS Elements imaging software is used to control acquisition and analysis of captured images. The software features include capabilities for single wavelength, multiple wavelength, and ratiometric mode fluorescence imaging.
主要功能包括：
6个激光线	目标
405 nm LD 20 mW 445 nm LD 20 MW 488 nm DPSS 70MW 514 nm DPSS 40 MW 561 nm DPSS 70mW 647 nm LD 125 mW	CFI计划Apo Lambda 4X CFI PLAN APO LAMBDA 10X CFI计划Apo Lambda 20X CFI计划荧光40x油NA1.30 WD 0.20MM CFI HP APO TIRF AC 100X油

Ivis Lumina

这Ivis Lumina Xris live, whole animal imaging system capable of performing longitudinal fluorescence, bioluminescence and X-ray scans onin vivoand离体主题。IVIS Lumina XR能够成像所有常见的荧光和生物发光的记者/染料，范围从绿色（〜488 nm）到近红外。该单元还包含用于实时动物研究的基于异氟烷的气体麻醉系统，尽管研究人员可以使用其他麻醉方法，只要它们得到了UT-IACUC的批准和记录。
主要功能包括：
Ivis Lumina Xr	X射线模块
荧光和生物发光的体内和体外成像高敏反固定的，后刷的1级CCD可在整个近红外光谱上提供高量子效率 Light-tight imaging chamber 8位置排放滤光轮 10个位置激发滤轮轮 5 filter wheel choices for a broad range of fluorescence applications 摄影图像的LED灯加热阶段以保持最佳体温 Automated motor controlled stage, filter wheel, lens position, and f-stop	Average X-ray acquisition in less than 10 seconds 辐射屏蔽机柜 Exceeds standards set by the US FDA Center for Devices and Radiological Health (21 CFR-1020.40) 与生物发光，荧光和照片叠加的自动图像集成
optical zoom lens attachment for close up and high-resolution X-ray

数据处理计算机：

Workstation 1: Nikon Advanced Super-Resolution Image Analysis

picture of the data processing computer

Workstation 2: General Image Analysis:

picture of Data processing computer

NIS Elements AR; Meta Morph; LIFA-FLIM; Living Image; Image J; Slidebook; AutoQuant, and Adobe Photoshop elements 2.0.