Education

Graduate School
Cell and Molecular Biology, University of Texas at Dallas, Richardson, Texas, 1988
Post Graduate School
University of Texas Southwestern Medical Center at Dallas, Department of Biochemistry, Dallas, Texas, 1988-1990

Areas of Interest

Clinical Interests

肺表面活性剂蛋白（SP）在肺功能和防御中起关键作用；SP-B和SP-C对于减少肺泡表面张力至关重要，而SP-A和SP-D是肺部宿主防御和炎症调节的关键。我们的研beplay苹果手机能用吗究兴趣围绕着了解表面活性剂蛋白在两种影响过早新生儿的严重病理中的作用。呼吸窘迫综合征（RDS）和坏死性小肠结肠炎（NEC）。

尽管由于早产过早而缺乏SP-B会导致肺功能障碍和RDS，但糖皮质激素的产前给药会通过增加SP-B mRNA稳定性来增加SP-B表达。我们已经确定了在人类SP-B mRNA 3'-非翻译区域中提高mRNA稳定性所必需的序列，现在正在鉴定与这些序列结合的蛋白质。我们的目标是定义这种调节机制，以便在不使用糖皮质激素的情况下设计疗法以加速早产婴儿的SP-B表达。

NEC是一种毁灭性的疾病往往伴随pre-maturity that results in partial or total destruction of the immature intestine via inappropriate bacterial colonization and rampant inflammation. In the lung, SP-A functions to reduce bacterial colonization and inflammation, and SP-A is expressed in the gastrointestinal tract. We found that SP-A reduces inflammatory cytokines in the intestine and colon of neonatal mice. In addition, we find that SP-A can reduce pathology in rodent models of NEC. SP-A appears to reduce levels of a key player in NEC, toll-like receptor-4 (TLR4) through increased proteasomal degradation. Our goal is to define the protective mechanism of SP-A in immature intestine and establish a link between the incidence of NEC and intestinal SP-A levels. These studies provide may a new paradigm in which the under-appreciated extra-pulmonary functions of SP-A may help protect the immature intestine. Defining the mechanism of this protection will allow us to design therapies that exploit the immunomodulatory properties of SP-A to prevent and treat NEC in prematurely-born infants.

beplay苹果手机能用吗研究兴趣

  1. Transcriptional and post-transcriptional regulation of pulmonary surfactant proteins in the lung and gastrointestinal tract.
  2. SP-A在调节TLR4中的作用在肠上皮。
  3. 表面活性剂蛋白与环境损伤的相互作用（RSV，颗粒物）
  4. Genetic influences on pulmonary type II epithelial cell biology and surfactant function

出版物

出版信息

  • Adibi S, Seferovic D, Tribble GD,Alcorn JL, Fakhouri WD. Surfactant Protein A and Microbiome Composition in Patients With Atraumatic Intraoral Lesions.Front Oral Health。2021; 2：663483。出版2021年4月22日。doi：10.3389/froh.2021.663483
  • Chetta Ke，Alcorn JL, Baatz JE, Wagner CL. Cytotoxic Lactalbumin-Oleic Acid Complexes in the Human Milk Diet of Preterm Infants. Nutrients. 2021;13(12):4336. Published 2021 Nov 30. doi:10.3390/nu13124336
  • Liu L, Liu X, Bi W,Alcorn JL。灵长类动物特异性的RNA结合蛋白（RBMXL3）参与人肺表面活性剂蛋白B（SP-B）mRNA稳定性的糖皮质激素调节。Am J Physiol Lung Cell Mol Physiol。2021;320(5):L942-L957. doi:10.1152/ajplung.00022.2020
  • Liu L, Aron CZ, Grable CM, et al. Surfactant protein A reduces TLR4 and inflammatory cytokine mRNA levels in neonatal mouse ileum.Sci Rep。2021; 11（1）：2593。发布于2021年1月28日。doi：10.1038/s41598-021-82219-y
  • Liu X, Liu L, Bi W,Alcorn JL。An internal amino-terminal FLAG-tag octapeptide alters oligomerization of expressed surfactant protein-A.Protein Expr Purif。2020;176:105727. doi:10.1016/j.pep.2020.105727
  • Adibi SS,Alcorn JL，Ono K，Lichtenberger LM。牙科患者唾液中表面活性剂脂质和蛋白质的性别和吸烟相关性。J Dent Maxillofac Surg。2018; 1（1）：67-70。doi：10.18314/jdms.v1i1.1385
  • Quan Y, Wang Z, Gong L, et al. Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling.Stem Cell Res Ther。2017;8(1):138. Published 2017 Jun 8. doi:10.1186/s13287-017-0586-2
  • 波西KL，Coustry F, Veerisetty AC, et al. Antisense Reduction of Mutant COMP Reduces Growth Plate Chondrocyte Pathology.Mol Ther。2017; 25（3）：705-714。doi：10.1016/j.ymthe.2016.12.024
  • Razvi党卫军,理查兹JB,马利克F,等。抵抗素德ficiency in mice has no effect on pulmonary responses induced by acute ozone exposure.Am J Physiol Lung Cell Mol Physiol。2015; 309（10）：L1174-L1185。doi：10.1152/ajplung.00270.2015
  • 波西KL，Coustry F, Veerisetty AC, Hossain M,Alcorn JL，Hecht JT。Antioxidant and anti-inflammatory agents mitigate pathology in a mouse model of pseudoachondroplasia.Hum Mol Genet。2015;24(14):3918-3928. doi:10.1093/hmg/ddv122
  • Quintanilla HD，Liu Y，Fatheree NY等。表面活性剂蛋白A的口服A-A在坏死性小肠结肠炎的实验模型中降低了病理。J Pediatr胃肠道营养。2015;60(5):613-620. doi:10.1097/MPG.0000000000000678
  • Yang W, Hu B, Wu W, et al. Alveolar type II epithelial cell dysfunction in rat experimental hepatopulmonary syndrome (HPS).PLOS ONE。2014;9(11):e113451. Published 2014 Nov 24. doi:10.1371/journal.pone.0113451
  • Fonseca L,Alcorn JL, Ramin SM, Vidaeff AC. Comparison of the effects of betamethasone and dexamethasone on surfactant protein A mRNA expression in human lung cells.J Matern Fetal Neonatal Med。28 2015;(11): 1239 - 1243。doi: 10.3109/14767058.2014。951623
  • 波西KL，Alcorn JL，Hecht JT。假核糖/comp - 从长凳上翻译成床边。Matrix Biol。2014;37:167-173. doi:10.1016/j.matbio.2014.05.006
  • Smith JA, Gaikwad A, Mosley S, et al. Utilization of an ex vivo human placental perfusion model to predict potential fetal exposure to carboplatin during pregnancy.Am J Obstet Gynecol。2014;210(3):275.e1-275.e2759. doi:10.1016/j.ajog.2013.12.013
  • 波西KL，Coustry F, Veerisetty AC, Liu P,Alcorn JL，Hecht JT。Chondrocyte-specific pathology during skeletal growth and therapeutics in a murine model of pseudoachondroplasia.J Bone Miner Res。2014;29(5):1258-1268. doi:10.1002/jbmr.2139
  • Yan Q, Quan Y, Sun H, et al. A site-specific genetic modification for induction of pluripotency and subsequent isolation of derived lung alveolar epithelial type II cells.干细胞。2014; 32（2）：402-413。doi：10.1002/stem.1570
  • Carreno CA,Alcorn JL, Vidaeff AC, Bishop KD, Blackwell SC, Sibai BM. Corticosteroids effect on caspase 3 expression in an in-vitro model of hypoxic brain cells.J Matern Fetal Neonatal Med。2013; 26（17）：1758-1761。doi：10.3109/14767058.2013.798287
  • Huang HW, Payne DE, Bi W, Pan S, Bruce SR,Alcorn JL。3'-非翻译区域中发夹结构的序列介导了人类肺表面活性剂蛋白B mRNA稳定性的调节。Am J Physiol Lung Cell Mol Physiol。2012;302(10):L1107-L1117. doi:10.1152/ajplung.00015.2012
  • Coustry F, Posey KL, Liu P,Alcorn JL，Hecht JT。D469del-COMP retention in chondrocytes stimulates caspase-independent necroptosis.Am J Pathol。2012; 180（2）：738-748。doi：10.1016/j.ajpath.2011.10.033
  • 波西KL，Coustry F, Veerisetty AC, Liu P,Alcorn JL，Hecht JT。Chop (Ddit3) is essential for D469del-COMP retention and cell death in chondrocytes in an inducible transgenic mouse model of pseudoachondroplasia.Am J Pathol。2012;180(2):727-737. doi:10.1016/j.ajpath.2011.10.035
  • Pedroza M, Schneider DJ, Karmouty-Quintana H, et al. Interleukin-6 contributes to inflammation and remodeling in a model of adenosine mediated lung injury.PLOS ONE。2011;6(7):e22667. doi:10.1371/journal.pone.0022667
  • Das A, Acharya S, Gottipati KR, et al. Thyroid transcription factor-1 (TTF-1) gene: identification of ZBP-89, Sp1, and TTF-1 sites in the promoter and regulation by TNF-α in lung epithelial cells [published correction appears in Am J Physiol Lung Cell Mol Physiol. 2011 Dec;301(6):L1003].Am J Physiol Lung Cell Mol Physiol。2011;301(4):L427-L440. doi:10.1152/ajplung.00090.2011
  • Chen MG, Atkins CL, Bruce SR, Khan AM, Liu Y,Alcorn JL。Infant formula alters surfactant protein A (SP-A) and SP-B expression in pulmonary epithelial cells.Pediatr Pulmonol。2011;46(9):903-912. doi:10.1002/ppul.21455
  • Pepiak DL,Alcorn JL, Atkins CL, Xue H, Colasurdo GN, Khan AM. Effects of infant formula on cell homeostasis and cytokine levels in an in vivo and in vitro murine aspiration model.Pediatr Pulmonol。2011;46(9):927-933. doi:10.1002/ppul.21444
  • Tillis CC，Huang HW，Bi W，Pan S，Bruce SR，Alcorn JL。人肺表面活性剂蛋白-B（SP-B）mRNA稳定性的糖皮质激素调节独立于活化的糖皮质激素受体。Am J Physiol Lung Cell Mol Physiol。2011;300(6):L940-L950. doi:10.1152/ajplung.00420.2010
  • 波西KL，Liu P, Wang HR, Veerisetty AC,Alcorn JL，Hecht JT。RNAi reduces expression and intracellular retention of mutant cartilage oligomeric matrix protein.PLOS ONE。2010;5(4):e10302. Published 2010 Apr 22. doi:10.1371/journal.pone.0010302
  • Wang D, Morales JE, Calame DG,Alcorn JL, Wetsel RA. Transplantation of human embryonic stem cell-derived alveolar epithelial type II cells abrogates acute lung injury in mice.Mol Ther。2010;18(3):625-634. doi:10.1038/mt.2009.317
  • 波西KL，Veerisetty AC, Liu P, et al. An inducible cartilage oligomeric matrix protein mouse model recapitulates human pseudoachondroplasia phenotype.Am J Pathol。2009;175(4):1555-1563. doi:10.2353/ajpath.2009.090184
  • Bruce SR, Atkins CL, Colasurdo GN,Alcorn JL。呼吸合胞病毒感染通过降低翻译效率来改变人肺上皮细胞中表面活性剂A的表达。Am J Physiol Lung Cell Mol Physiol。2009;297(4):L559-L567. doi:10.1152/ajplung.90507.2008
  • Alcorn JL, Merritt TM, Farach-Carson MC, Wang HH, Hecht JT. Ribozyme-mediated reduction of wild-type and mutant cartilage oligomeric matrix protein (COMP) mRNA and protein.RNA。2009;15(4):686-695. doi:10.1261/rna.1335909
  • Huang HW，Bi W，Jenkins GN，Alcorn JL。Glucocorticoid regulation of human pulmonary surfactant protein-B mRNA stability involves the 3′-untranslated region.Am J Respir Cell Mol Biol。2008;38(4):473-482. doi:10.1165/rcmb.2007-0303oc
  • Vidaeff AC，Ramin SM，Gilstrap LC 3rd，Bishop KD，Alcorn JL。孕酮对HeLa细胞中细胞因子刺激的核因子-KAPPAB信号的影响。J Matern Fetal Neonatal Med。2007;20(1):23-28. doi:10.1080/14767050601128019
  • Brunelli L, Cieslik KA,Alcorn JL, Vatta M, Baldini A. Peroxisome proliferator-activated receptor-delta upregulates 14-3-3 epsilon in human endothelial cells via CCAAT/enhancer binding protein-beta.Circ Res。2007;100(5):e59-e71. doi:10.1161/01.RES.0000260805.99076.22
  • Merritt TM，Bick R，Poindexter BJ，Alcorn JL，Hecht JT。Unique matrix structure in the rough endoplasmic reticulum cisternae of pseudoachondroplasia chondrocytes.Am J Pathol。2007; 170（1）：293-300。doi：10.2353/ajpath.2007.060530
  • Merritt TM,Alcorn JL，Haynes R，Hecht JT。突变软骨寡聚基质蛋白在人软骨细胞中的表达诱导假核糖表型。J Orthop Res。2006; 24（4）：700-707。doi：10.1002/jor.20100
  • Alcorn JL，Stark JM，Chiappetta CL，Jenkins G，Colasurdo GN。RSV感染对培养的人类II型细胞中肺表面活性剂蛋白SP-A的影响：对SP-A mRNA和蛋白质的对比后果。Am J Physiol Lung Cell Mol Physiol。2005;289(6):L1113-L1122. doi:10.1152/ajplung.00436.2004
  • Phokela SS, Peleg S, Moya FR,Alcorn JL。Regulation of human pulmonary surfactant protein gene expression by 1alpha,25-dihydroxyvitamin D3.Am J Physiol Lung Cell Mol Physiol。2005;289(4):L617-L626. doi:10.1152/ajplung.00129.2004
  • Romero EJ, Moya FR, Tuvim MJ,Alcorn JL。Interaction of an artificial surfactant in human pulmonary epithelial cells.Pediatr Pulmonol。2005; 39（2）：167-177。doi：10.1002/ppul.20166
  • Stark JM, Khan AM, Chiappetta CL, Xue H,Alcorn JL, Colasurdo GN. Immune and functional role of nitric oxide in a mouse model of respiratory syncytial virus infection.J Infect Dis。2005; 191（3）：387-395。doi：10.1086/427241
  • Vidaeff AC, Ramin SM, Gilstrap LC 3rd,Alcorn JL。Characterization of corticosteroid redosing in an in vitro cell line model.Am J Obstet Gynecol。2004;191(4):1403-1408. doi:10.1016/j.ajog.2004.06.049
  • Vidaeff AC, Ramin SM, Gilstrap LC 3rd,Alcorn JL。在人肺细胞中，替克米松诱导的表面活性剂蛋白B表达的体外定量。J Matern Fetal Neonatal Med。2004;15(3):155-159. doi:10.1080/14767050410001668248
  • Ramin SM，Vidaeff AC，Gilstrap LC 3rd，KD Bishop KD，Jenkins GN，Alcorn JL。The effects of dexamethasone and betamethasone on surfactant protein-B messenger RNA expression in human type II pneumocytes and human lung adenocarcinoma cells.Am J Obstet Gynecol。2004; 190（4）：952-959。doi：10.1016/j.ajog.2004.01.015
  • Alcorn JL，伊斯兰教义，年轻PP，门德森CR。糖皮质激素对肺II型细胞中SP-A基因表达的抑制是通过TTF-1结合元件介导的。Am J Physiol Lung Cell Mol Physiol。2004;286(4):L767-L776. doi:10.1152/ajplung.00280.2003
  • Mustafa SB，Digeronimo RJ，Petershack JA，Alcorn JL, Seidner SR. Postnatal glucocorticoids induce alpha-ENaC formation and regulate glucocorticoid receptors in the preterm rabbit lung.Am J Physiol Lung Cell Mol Physiol。2004;286(1):L73-L80. doi:10.1152/ajplung.00342.2002
  • Shlykov SG, Yang M,Alcorn JL，Sanborn BM。人肌层细胞中的电容阳离子进入和HTRPC3过表达的增强。Biol Reprod。2003;69(2):647-655. doi:10.1095/biolreprod.103.015396
  • Razeghi P, Young ME,Alcorn JL, Moravec CS, Frazier OH, Taegtmeyer H. Metabolic gene expression in fetal and failing human heart.循环。2001; 104（24）：2923-2931。doi：10.1161/hc4901.100526