Education

M.D.
Capital Institute of Medicine, Beijing, China, 1986

Areas of Interest

Research Interests

EPAC-mediated cell signaling: physiological functions and roles in human diseases

Research Information

EPAC-mediated cell signaling: physiological functions and roles in human diseases

Adenosine 3′,5′-cyclic monophosphate (cAMP)is the prototypic second messengerthat regulates a multitude of important biological processes under both physiological and pathological conditions, including diabetes, heart failure, and cancer. In eukaryotic cells, the effects of cAMP are mainly mediated by two ubiquitously expressed intracellular cAMP receptors, the classic cAMP-dependent protein kinase (PKA) and exchange protein directly activated by cAMP (EPAC).My research focus is on elucidating the physiological functions of EPAC proteins and their roles in human diseases.

我们应用遗传和药理方法来了解EPAC蛋白。我们已经开发了高通量屏幕分析，成功鉴定出了新型EPAC特异性抑制剂。与UCSD的Chen博士合作，我们生成了EPAC敲除小鼠模型，这使我们能够揭示EPAC信号传导与多种病理状况的发展有关。与EPAC特异性抑制剂和淘汰小鼠模型的结合在一起，我们处于回答重要生物学问题的理想位置and to develop potential therapeutics for the treatment of human diseases where EPAC proteins are implicated.

Publications

Publication Information

参考

  • Almahariq M,梅，and Cheng X.（2014）。营地传感器EPAC蛋白质和能量稳态。趋势Endocrin Metabol,25:60-71.
  • Tao T,梅, Agrawal A, Peters CJ, Ksiazek T, Cheng X*, and Tseng CT.* (2014). Blocking of Exchange Proteins Directly Activated by cAMP (Epac) Leads to Reduced Replication of Middle East Respiratory Syndrome-Coronavirus.J Virology,Epub ahead of print Jan. 22.
  • Gong B*，Shelite T，梅，Ha T，Xu G，Chang Q，Hu Y，Wakamiya M，Ksiazek TG，Boor PJ，Bouyer R，Popov V，Chen J，Walker DH和Cheng X.* (2013).Exchange protein directly activated by cAMP plays critical role in fatal rickettsioses.Proc Acad Natl Sci, USA,110:19615-19620.
  • Almahariq M, Tsalkova T,梅C, Chen H, Zhou J, Sastry SK, Schwede F, and Cheng X. (2013). A novel EPAC-specific inhibitor suppresses pancreatic cancer cell migration and invasion.Molec Pharm,83:122-128.
  • Yan J,梅C, Cheng HQ, Lao DH, Hu Y, Wei J, Patrikeev I, Hao D, Stutz SJ, Dineley KT, Motamedi M, Hommel JD, Cunningham KA, Chen J*, and Cheng X*. (2013). Enhanced leptin sensitivity, reduced adiposity and improved glucose homeostasis in mice lacking of exchange protein directly activated by cAMP isoform 1.Molec Cell Bio,33：918-926。
  • Chen H, Tsalkova T,梅C, Cheng X.*, and Zhou J.* (2013). Identification and characterization of small molecules as potent and specific EPAC antagonists.J Med Chem,56:952-962.
  • Peng Z, Ji Z,梅C, Lu M, Zhou W, Ou Y, Cheng X. (2013). Lithium Inhibits Tumorigenic Potential of PDA Cells through Targeting Hedgehog-GLI Signaling Pathway.PLOS ONE, 8, e61457.
  • tsalkova t，梅C, Li Sheng, Chepurny OG, Liu T, Woods Jr., VL, Holz GG, and Cheng X. (2012). Isoform-specific antagonists of exchange protein directly activated by cAMP.Proc Acad Natl Sci, USA,109:18613-18618.
  • tsalkova t，梅C, and Cheng X. (2012). A Fluorescence-based High-throughput Assay for the discovery of Exchange Protein directly Activated by Cyclic AMP (EPAC) Antagonists.PLOS ONE,7(1):e30441.
  • Chen H, Tsalkova T,梅C, Hu Y, Cheng X.*, and Zhou J.* (2012). 5-Cyano-6-oxo-1,6-dihydro-pyrimidines as Potent Antagonists Targeting Exchange Proteins Directly Activated by cAMP.Bioorganic & Medicinal Chem Lttrs.22：4038-4043。
  • Ji Z,梅C, and Cheng X. (2010). Epac, not PKA catalytic subunit, is required for 3T3-L1 preadipocyte differentiation.Front Biosci (Elite Ed).2:392-398.
  • Ji Z,梅C, Miller AL, Thompson EB, and Cheng X. (2008). Protein kinase A (PKA) isoform RIIβ mediates the synergistic killing effect of cAMP and glucocorticoid in acute lymphoblastic leukemia cells.J Biol Chem，283：21920-21925。
  • Cheng X，Ji Z，Tsalkova T和梅C.(2008). Epac and PKA: a tale of two intracellular cAMP receptors.Acta Biochimica et Biophysica Sinica.40:651-662.
  • 年轻TW，梅C，Rosen DG，Yang G，Li N，Liu J和Cheng X.（2007）。功能蛋白质组学揭示了人类上皮卵巢癌中肿瘤易感性基因101蛋白的上调。Mol Cell Proteomics.6:294-304.
  • Ji Z,梅C, Xie J, and Cheng X. (2007). Oncogenic KRAS suppresses GLI1 degradation and activates hedgehog signaling pathway in pancreatic cancer cells.J Biol Chem，282:14048-14055.
  • 年轻TW，Rosen DG,梅, Li N, Liu J, and Cheng X. (2007). Up-regulation of Tumor Susceptibility Gene 101 Conveys poor Prognosis through Suppression of p21 Expression in Ovarian Cancer.Clinic Can Res,13:3848-3854.
  • Ji Z,梅C, Johnson BH, Thompson EB, and Cheng X. (2007). PKA, not Epac, suppresses hedgehog activity and regulates glucocorticoid sensitivity in acute lymphoblastic leukemia cells.J Biol Chem，282:37370-37377.