教育

BA
心理生物学，加州大学洛杉矶分校，1973年
多发性硬化症
Biology/Biochemistry, UC Santa Barbara, 1975
PhD
Microbiology/Immunology, UCLA, 1980
博士后
UC San Diego, 1980-1982

Areas of Interest

Research Interests

细菌发病机理和宿主病原体相互作用

beplay苹果手机能用吗研究信息

Science is revealing the truth about nature.

细菌发病机理和宿主病原体相互作用

Our research is driven by an interest in bacterial pathogenesis and its relationship with microbial physiology, structure, and genetics.Treponema pallidum(the causative agent of syphilis) andBorrelia burgdorferi(which causes Lyme disease) are spirochetes that cause chronic, multisystemic diseases. These organisms can invade almost any tissue and survive and cause disease in the host for years to decades, and thus serve as important examples of persistent, invasive pathogens.

Long-term in vitro culture ofTreponema pallidum

The culture ofT. pallidum自1905年鉴定为梅毒的原因以来，体外一beplay苹果手机能用吗直是研究目标。我们的小组最近描述了一个支持长期乘法的系统T. pallidumin a tissue culture system (Edmondson et al., 2018). We have now maintained exponential growth ofT. pallidumfor over three years and have embarked on an NIH-funded project to 1) optimize the tissue culture system, 2) delineate nutritional requirements and growth characteristics, with the goal of developing an axenic (cell-free) culture system, and 3) investigate the possibility of mutational analysis ofT. pallidum。Other potential applications include the direct culture ofT. pallidum从患者标本和该系统用于抗菌敏感性测试。

莱姆病发病机理的分子遗传学

分子遗传技术，例如转座子诱变（包括标记为标记的诱变），PCR，Luminex®的基于Luminex®的高通量筛选程序和反向基因组学与感染性研究一起使用，以鉴定B. Burgdorferigenes and gene products required for infectivity in a mouse model. Using such approaches, our group identified and characterized thevlsantigenic variation system ofB. Burgdorferi这是一种类似于可变的重大蛋白质系统的精细机制。我们还利用了一种全球方法来鉴定通过使用高通量方法（包括TN-SEQ）筛选转座子突变库来鉴定对莱姆病发病机理中重要的毒力决定因素的其他基因。

Ultrastructure of pathogenic spirochetes

Cryo-electron tomography, a relatively new electron microscopy technique that creates 3D images of bacteria, is being utilized in collaborative studies with Dr. Jun Liu at Yale University to elucidate the architecture of the flagellar motor and other structural elements of these organisms.

Publications

Publication Information

Selected Publications (Click这里for current PubMed listings)

  • Leimer N, Wu X, Imai Y, Morrissette M, Pitt N, Favre-Godal Q, Iinishi A, Jain S, Caboni M, Leus IV, Bonifay V, Niles S, Bargabos R, Ghiglieri M, Corsetti R, Krumpoch M, Fox G, Son S, Klepacki D, Polikanov YS, Freliech CA, McCarthy JE, Edmondson DG, Norris SJ, D’Onofrio A, Hu LT, Zgurskaya HI, Lewis K. A selective antibiotic for Lyme disease. Cell. 2021 Sep 30:S0092-8674(21)01058-8. doi: 10.1016/j.cell.2021.09.011. PMID: 34619078
  • De Lay BD，Cameron TA，De Lay NR，Norris SJ，Edmondson DG。2021.比较转录曲线的比较Treponema pallidumduring experimental infection of rabbits and in vitro culture: Highly similar, yet different. PLoS Pathog Sep 27;17(9):e1009949. doi: 10.1371/journal.ppat.1009949.PMC8525777
  • Edmondson DG, Norris SJ. 2021. In vitro cultivation of the syphilis spirochete,Treponema pallidum。当前协议期刊。2021年2月; 1（2）：E44。doi：10.1002/cpz1.44。PMCID：PMC7986111
  • Edmondson DG, DeLay BD, Kowis LE, Norris SJ. 2021. Parameters affecting continuousin vitroculture ofTreponema pallidumstrains. mBio. doi: 10.1128/mBio.03536-20. PMID: 32895555
  • Coburn，J，Garcia B，Hu LT，Jewett MW，Kraiczy P，Norris，SJ，Skare J.2020。莱姆病发病机理。Curr问题MOL BIOL 12月23日； 42：473-518。doi：10.21775/cimb.042.473。PMCID：PMC8046170
  • Zhang Y，Chen T，Raghunandanan S，Xiang X，Yang J，Liu Q，Edmondson DG，Norris SJ，Yang XF，LouY.2020。Yebc调节可变的表面抗原VLSE表达，是宿主免疫在宿主中所必需的Borrelia burgdorferi。PLoS Pathog 16(10):e1008953. doi: 10.1371/journal.ppat.1008953. PMCID:PMC7584230
  • Hahn H，Anderson P，Lu Z，Danner R，Zhou Z，Hyun N，Gao L，Lin T，Norris SJ，CoburnJ.2020。BBB07对，但对，但对，但不是必需的，Borrelia burgdorferiinfection in mice. Microbiology (Reading). 2020 Oct;166(10):988-994. doi: 10.1099/mic.0.000972. PMCID:PMC7660918
  • Chang Y, Zhang K, Carroll BL, Zhao X, Charon NW, Norris SJ, Motaleb MA, Li C, Liu J. 2020. Molecular mechanism for rotational switching of the bacterial flagellar motor. Nature Struct Molec Biol, Nat Struct Mol Biol. 2020 Nov;27(11):1041-1047. doi: 10.1038/s41594-020-0497-2. PMID: 32718967
  • Edmondson DG, Wormser GP, Norris SJ. 2020.In vitro易感性Treponema pallidum亚种。pallidum去多西环素。抗小动物化学剂64：e00979-20。PMID：32718967
  • Medina-Pérez DN, Wager B, Troy E, Gao L, Norris SJ, Lin T, Hu L, Hyde JA, Lybecker M, Skare JT. 2020. The intergenic small non-coding RNAittAis required for optimal infectivity and tissue tropism inBorrelia burgdorferi。PLoS Pathog. 16:e1008423. PMC: 7224557
  • Chang Y，Moon KH，Zhao X，Norris SJ，Motaleb MA，Liu J.2019。对鞭毛定子互动的结构见解。Elife 8：E48979。doi：10.7554/elife.48979。PMID：31313986
  • Phelan JP，Kern A，Ramsey ME，Lundt Me，Sharma B，Lin T，Gao L，Norris SJ，Hyde JA，Skare JT，Hu Lt。2019年。全基因组屏幕确定了所需的新基因Borrelia burgdorferisurvival in itsIxodestick vector. PLoS Pathog.15:e1007644. PMID: 31086414
  • Qin Z, Tu J, Lin T, Norris SJ, Li C, Motaleb MA, Liu J. 2018. Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex. PLoS Biol16:e3000050. PMC: 6248999
  • 诺里斯·SJ。2019年。隐藏在视力中：人类的结肠螺旋病。（评论）J细菌。201：e00465-19。doi：10.1128/jb.00465-19。PMC6779462
  • 诺里斯·SJ。2018.赶上莱姆病抗原变异的计算。（评论）趋势微生物。26（8）：644-645。doi：10.1016/j.tim.2018.05.017。PMID：29903419。Link
  • Edmondson，DG，Hu B，Norris SJ。2018。梅毒螺旋体的长期体外培养，Treponema pallidum。MBio. 9(3). pii: e01153-18. doi: 10.1128/mBio.01153-18. PMID: 29946052Link
  • Arora N, J Verena, GJ Schuenemann, A Peltzer, A Seitz, A Herbig, M Strouhal, L Grillová, L Sánchez-Busó, D Kühnert, KI. Bos, N Juricevic, L R Davis, L Paštěková, S Bruisten, P Komericki, P French, P Grant, MA Pando, LG Vaulet, M R Fermepin, A Martinez, A Centurion Lara, L Giacani, SJ Norris, D Smâjs, PP Bosshard, F González-Candelas, K Nieselt, J Krause and HC Bagheri. 2016. Origin of modern syphilis and emergence of a pandemic cluster. Nature Microbiology 5;2:16245. doi: 10.1038/nmicrobiol.2016.245. PMID: 27918528
  • Edmondson DG，Prabhakaran S，Norris SJ，Ullmann AJ，Piesman J，Dolan M，Probst C，Radzimski C，StöckerW，StöckerW，Komorowski L.增强同性恋borlelia Borrelia borgdoria burgdorferi burgdorferi burgdorferi burgdorferi burgdorferi burgdorferi burgdorferi burgdorferi burgdorferi burgdorferi burgdorferi protferi protein protein蛋白C. Clincine C. Clincine Clincine Clincine Clincine clincine clincine。2016年10月12日。PII：CVI.00306-16。PMCID：PMC5216437
  • Bourret TJ, Lawrence KA, Shaw JA, Lin T, Norris SJ, Gherardini FC. 2016. The nucleotide excision repair pathway protects Borrelia burgdorferi from nitrosative stress in Ixodes scapularis ticks. Front Microbiol. 2016 Sep 7;7:1397. doi: 10.3389/fmicb.2016.01397. PMCID: PMC5013056
  • Troy EB，Lin T，Gao L，Lazinski DW，Lundt M，Camilli A，Norris SJ，Hu LT。Borrelia burgdorferi的碳水化合物利用率和脊椎动物感染性的全球TN-SEQ分析。摩尔微生物。2016年9月; 101（6）：1003-23。doi：10.1111/mmi.13437。PMCID：PMC5028225
  • Chu, C-Y, PE Stewart, A Bestor, B Hanson, T Lin, L Gao, SJ Norris, and PA Rosa. 2016. Function of the Borrelia burgdorferi FtsH homolog is essential for viability both in vitro and in vivo, and independent of HflK/C. mBio 7(2). pii: e00404-16. doi: 10.1128/mBio.00404-16. PMC4850261
  • Khajanchi BK E Odeh, LGao, M Jacobs, MT Philipp, T Lin, and SJ Norris. 2016. Phosphoenolpyruvate phosphotransferase system components modulate gene transcription and virulence of Borrelia burgdorferi. Infect Immun 84:754 –764. doi:10.1128/IAI.00917-15. PMC4771366
  • Lin, T., L. Gao, X. Zhao, J. Liu, and S. J. Norris. 2015. Mutations in the Borrelia burgdorferi flagellar type III secretion system genes fliH and fliI profoundly affect spirochete flagellar assembly, morphology, motility, structure and cell division. mBio 6(3):e00579-15. doi:10.1128/mBio.00579-15, PMC4436065.
  • Čejková, D. M. Strouhal, S.J. Norris, G. M. Weinstock and D.Šmajs. 2015. A retrospective study on genetic heterogeneity within Treponema Strains: Subpopulations are genetically distinct in a limited number of positions. PLoS Negl Trop Dis. 2015 Oct 5;9(10):e0004110. doi: 10.1371/journal.pntd.0004110. PMID: 26436423.
  • Darkoh，C.，H。L. Dupont，S。J. Norris和H. B. Kaplan。2015年。艰难梭菌的毒素合成通过法定信号传导机制严格调节。MBIO 6（2）：E02569-14。doi：10.1128/mbio.02569-14，PMC4453554。
  • 诺里斯·SJ。2014. vls antigenic variation systems of Lyme disease Borrelia: eluding host immunity through both random, segmental gene conversion and framework heterogeneity. Microbiol Spectrum 2(6):MDNA3-0038-2014. doi:10.1128/microbiolspec. MDNA3-0038-2014, PMC4480602.
  • Lin T, Troy EB, Hu LT, Gao L, Norris SJ。2014年。转座子诱变是一种改善对理解的方法Borrelia发病机理和生物学。前细胞感染微生物4：63.抽象的
  • Zhao X, Norris SJ, Liu J. 2014. Molecular architecture of the bacterial flagellar motor in cells. Biochemistry. 53:4323-4333. PMID: 24697492 Abstract
  • Zhao X, Zhang K, Boquoi T, Hu B, Motaleb MA, Miller KA, James ME, Charon NW, Manson MD, Norris SJ, Li C, Liu J. 2013. Cryoelectron tomography reveals the sequential assembly of bacterial flagella in Borrelia burgdorferi. Proc Natl Acad Sci (USA) 110: 14390-14395. Abstract
  • Chaconas G,诺里斯SJ。2013.和平共处amongst Borrelia plasmids: Getting by with a little help from their friends? Plasmid 70: 161-167. Abstract
  • Troy EB，Lin T，Gao L，Lazinski DW，Camilli A，Norris SJ，Hu Lt。2013年。了解在感染过程中使用大量平行测序在感染过程中疏cor伯氏菌传播的障碍。感染免疫81：2347-2357。抽象的
  • Lin T, Gao L, Zhang C, Odeh E, Jacobs MB, Coutte Lc, Chaconas G, Philipp MT, Norris SJ. 2012. Analysis of an ordered, comprehensive STM mutant library in infectious Borrelia burgdorferi: Insights into the genes required for mouse infectivity. PLoS ONE 7: e47532. Abstract
  • Norris, S J., J. A. Howell, E. A. Odeh, T. Lin, L. Gao, and D. G. Edmondson. 2011. High-throughput plasmid content analysis of Borrelia burgdorferi B31 using Luminex multiplex technology. Appl. Env. Microbiol. 77:1483-1492. Abstract

附加信息

Academic Appointments

Univ. of California at San Diego School of Medicine, Department of Pathology
  • Assistant Research Pathologist, 1981‑1982.
UTHealth Medical School, Dept. of Pathology and Laboratory Medicine
  • Assistant Professor, 1982‑1989.
  • 副教授，1989- 1996年。
  • Cross Appointment, Dept. of Microbiology and Molecular Genetics , 1990-.
  • Professor, 1996-.
  • Vice Chair for Research, 1998-.
  • 罗伯特·格里尔（Robert Greer）生物医学科学教授，2003年。

荣誉和奖励

  • Fellow, American Academy of Microbiology, 1993-.
  • Faculty Mentor of the Year, University of Texas Medical School, 2000.
  • Karin Ippen-Ihler讲座，得克萨斯州农工大学，2003年。
  • 多个机构项目的总理企业家和创新奖，得克萨斯大学系统，2007年。
  • Regents Outstanding Teacher Award, University of Texas Health Science Centers, 2013.
  • Member, The University of Texas Kenneth I. Shine Academy of Health Science Education, 2014-.

Other Professional Activities

  • Reviewer, National Institutes of Health, Institute of Allergy and Infectious Diseases (over 40 study sections and 18 teleconference review panels)
  • 经常会员，细菌学和真菌学I研究部分，1999-2003
  • Chair, Infectious Diseases & Microbiology-B Special Emphasis Panel for R21 and R03 applications (5 sessions, 2014-2019).
  • Vice Chair and Chair, Gordon Research Conference on Biology of Spirochetes, 1996-2000.
  • Member, Board of Editors,MBIO，2013-。
  • Member, Editorial Board,Infection and Immunity, 1994-.

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