青春汤
教授
休斯敦德克萨斯大学健康科学中心
麦戈文医学院
分子医学研究所
代谢和退化性疾病中心
我们的主要研究兴趣是阐明beplay苹果手机能用吗神经ocircuits and behavior. We aims to map novel functionally important neurocircuits and understand the physiology of known neurocircuits. Specifically, we are interested in disease conditions of abnormal feeding and related emotional states, and obesity development. The current obesity epidemic and its associated metabolic syndrome have imposed unprecedented challenges to society and medicine, but with no apparent effective therapeutics. Our research is directed to understand the fundamental mechanistic insights on key driving causes for defective feeding and body weight regulation, therefore providing conceptual and effective targets for prevention and treatment of eating disorders, obesity and its associated diabetes.
为了实现我们的目标，我们将各种动物模型与最先进的技术结合使用，包括电生理学，光遗传学，化学遗传学，体内活体Ca2+成像和各种神经跟踪方法。Cre-lox P小鼠遗传学用于实现大脑中神经元特异性的操作。具有表现出CRE依赖性表达的基因的各种腺体相关病毒载体（AAV）将传递到表达CRE的神经元的特定大脑区域，从而实现外国工具基因的神经元表达。示例外源基因包括激活或抑制神经元的特定通道。此外，基于病毒的跟踪用于绘制特定的神经投影及其对生理和行为的影响。此外，我们正在探索使用CRISPR/CAS9技术来实现成年小鼠的神经元特异性基因缺失。我们还将将新可用的FLPO系和FLPO依赖性AAV向量与现有的CRE-LoxP系统相结合，以实现更特定的靶向和跟踪功能性神经电路。这些先进的技术确保我们的研究有效，结论是有见地的。
Students will have opportunities to map neurocircuits and behavior, and related metabolic studies using animal models with neuron-specific manipulations. Optogenetics, chemogenetics, in vivo Ca2+ imaging, neural tracing, Immunocytochemistry, in situ hybridization, animal physiology and animal surgery are routinely used in the lab. Tutorial is available to highly motivated students.
revised 1/13/2020
教育与培训
博士- 纽约州立大学医疗中心 - 2003年