玛格丽达阿尔伯克基阿尔梅达·桑托斯
助理教授
The University of Texas MD Anderson Cancer Center
Department of Epigenetics and Molecular Carcinogenesis
我的科学兴趣是专注于复制的stress/DNA damage response and epigenetic regulators in stem cell cancer. Current studies in my laboratory focus on the hematopoietic system, since 1) it is a well-established system for adult stem cells studies and 2) the dynamic nature of the hematopoietic system places it in a vulnerable position with respect to genomic damage during DNA replication. Replicative stress can be defined as a slowing or stalling of replication fork progression and a source of spontaneous DNA lesions that drives genomic instability. “Oncogene-induced” replicative stress is a major driving force of hematological cancers. Aberrant oncogene expression induces precocious entry into S phase and perturbs replication fork progression, triggering the DNA damage response. The classical view of the DNA damage response (DDR) postulates that DDR is a crucial tumorigenesis barrier in early stages of cancer development, and a selective pressure that favors malignant clones with defects in DNA repair factors. My recent work showed that DNA damage induces the differentiation of leukemic stem-like cells in acute myeloid leukemia (AML) harboring the MLL-AF9 oncogene, thus uncovering an unexpected tumor-promoting role of genome guardians in enforcing the oncogene-induced differentiation blockade in AML (Santos et al., Nature 2014).
我的实验室中的目前研究探讨了使用小鼠模型，下一代测序以及各种DNA损伤治疗和测定的AML和其他攻击性血液学恶性肿瘤中“ DNA损伤引起的类似茎状癌细胞分化”的概念。为了促进白血病干细胞的有效分化，应阐明哪种DNA损伤反应蛋白是针对这些癌症的新疗法的下一个重要步骤。
与我们一起的教程将提供小鼠工作的经验（体内白血病研究），流式细胞仪，下一代测序（CHIP-SEQ，RNA-SEQ和EXOME测序），免疫荧光和细胞学。
教育与培训
PhD, University of Lisbon Medical School, 2007
beplay苹果手机能用吗研究信息
癌症干细胞中的DNA损伤和表观遗传学