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Walker博士是Uthealth Houston的McGovern医学院的微生物学和分子遗传学系的助理教授。Walker博士在与Drs完成博士后培训后，于2019年加入该部门。密苏里州圣路易斯华盛顿大学医学院的Scott Hultgren和Michael Caparon。

Dr. Walker received her Ph.D. from the Department of Microbiology and Immunology at the University of Iowa after completing her doctoral thesis work.

Walker博士是德克萨斯州新星奖（2019年）的获得者。

Education

博士后研究员
华盛顿大学医学院
博士微生物学
The University of Iowa

Research Information

沃克实验室（Walker Lab）的重点是理解决定慢性感染的发作，过程和结果的宿主 - 病原体相互作用。我们的工作使用医疗设备的感染作为慢性疾病的模型。通过定义细菌和宿主机制促进这些顽固感染，我们试图开发新型的抗生素保留疗法，这些抗生素较高的疗法可以有效地治疗常见和昂贵的疾病。我们使用多学科方法来融合基础科学，模型系统和患者样本的使用来提出以下问题：

1.医疗设备如何感染？

每年都会放置数百万个医疗设备，并且由于它们在改善生活质量方面的功效，预计它们的使用将增加。但是，医疗设备的感染是一种常见的，可怕的并发症。尽管有率，但尚不清楚设备感染是由于医院环境中的细菌还是通过人自己的微生物组污染引起的。另外，某些细菌或毒力因素是否更有可能引起症状性与无症状感染，尚不清楚。我们与医生紧密合作，并结合使用模型系统，患者样本和基因组学来了解细菌如何启动装置定植，并将这些发现转化为更好的监视，预防和治疗策略。

2。How do medical devices render people susceptible to infection?

It is a well-known phenomenon that medical devices render people susceptible to atypical or “less pathogenic” bacteria, yet the mechanisms responsible remain largely unknown. Our recent studies indicate the device itself induces inflammation, which may prevent the host from mounting an effective response against these “less pathogenic” bacteria, allowing them to cause disease. To define these interactions, we are combining the use of model systems and patient samples to understand the inflammatory response to devices with and without infection. This work involves immunology, microbiology, and biochemistry for the identification of biomarkers that predict infection risk and the development of better device materials that reduce infections.

3. What are the bacterial mechanisms that facilitate medical device infections?

Staphylococci are the primary cause of device infections and form recalcitrant biofilms on the device surface. Our group recently discovered that staphylococci use different adhesins to attach to various host proteins coating device surfaces to initiate biofilm formation. This work uses bacterial genetics and molecular microbiology to understand the host-pathogen-device interactions that facilitate infection to develop novel antibiotic-sparing treatment strategies.

出版物

出版信息

沃克JN，Pinkner CL，Lynch AJL，Ortbal S，Pinkner JS，Hultgren SJ，Myckatyn TM。Deposition of Host Matrix Proteins on Breast Implant Surfaces Facilitates Staphylococcus Epidermidis Biofilm Formation: In Vitro Analysis.Aestshet Surg j。20202月17日；40（3）:281-295。doi：10.1093/asj/sjz099。PubMed PMID: 30953053。

沃克JN, Hanson BM, Pinkner CL, Simar SR, Pinkner JS, Parikh R, Clemens MW, Hultgren SJ, Myckatyn TM.Insights into the Microbiome of Breast Implants and Periprosthetic Tissue in Breast Implant-Associated Anaplastic Large Cell Lymphoma.Sci代表。20197月17日；9（1）:10393。doi: 10.1038/s41598-019-46535-8.PubMed PMID: 31316085;PubMed Central PMCID: PMC6637124。

沃克JN, Poppler L, Pinkner CL, Hultgren SJ, Myckatyn T.Establishment and Characterization of Bacterial Infection of Breast Implants in a Murine Model.Aestshet Surg j。20191月1日；。doi：10.1093/asj/sjz190。[EPUB在印刷前]PubMed PMID：31259380。

沃克JN，Pinkner CL，Pinkner JS，Hultgren SJ，Myckatyn TM。The Detection of Bacteria and Matrix Proteins on Clinically Benign and Pathologic Implants.塑料重新施加外侧环球打开。2019Feb;7（2）:e2037。doi：10.1097/gox.0000000000002037。eCollection2019Feb.PubMed PMID：30881821;PubMed Central PMCID：PMC6416121。

沃克JNFlores-Mireles AL Pinkner CL,施赖伯HL 4日Joens MS, Park AM, Potretzke AM, Bauman TM, Pinkner JS, Fitzpatrick JAJ, Desai A, Caparon MG, Hultgren SJ.导管插入改变膀胱生态以增强Staphylococcus aureusinfection of the urinary tract.Proc Natl Acad Sci U S A。2017Oct 10;114（41）：E8721-E8730。doi: 10.1073/pnas.1707572114.epub20179月25日。PubMed PMID: 28973850;PubMed Central PMCID: PMC5642702。

Flores-Mireles AL,沃克JN，Potretzke A，Schreiber HL 4，Pinkner JS，Bauman TM，Park AM，Desai A，Hultgren SJ，Caparon MG。基于抗体的肠球菌导管相关尿路感染的治疗。mBio。2016Oct 25;7（5）。doi: 10.1128/mBio.01653-16.PubMed PMID：27795399;PubMed Central PMCID：PMC5080383。

Flores-Mireles AL,沃克JN, Bauman TM, Potretzke AM, Schreiber HL 4th, Park AM, Pinkner JS, Caparon MG, Hultgren SJ, Desai A.Fibrinogen Release and Deposition on Urinary Catheters Placed during Urological Procedures.J Urol。2016八月;196（2）:416-421。doi：10.1016/j.juro.2016.01.100。epub2016Jan 28.PubMed PMID：26827873;PubMed Central PMCID: PMC4965327。

Flores-Mireles AL,沃克JN, Caparon M, Hultgren SJ.尿路感染：流行病学，感染机制和治疗方案。NAT Rev Microbiol。2015May;13（5）：269-84。doi: 10.1038/nrmicro3432.epub2015Apr 8.Review.PubMed PMID: 25853778;PubMed Central PMCID: PMC4457377。

沃克JN, Crosby HA, Spaulding AR, Salgado-Pabón W, Malone CL, Rosenthal CB, Schlievert PM, Boyd JM, Horswill AR.金黄色葡萄球菌ARLRS两组分系统是凝集和发病机理的新型调节剂。PLoS Pathog。2013;9(12):e1003819。doi：10.1371/journal.ppat.1003819。epub201312月19日。PubMed PMID：24367264;PubMed Central PMCID: PMC3868527。

沃克JN, Horswill AR.A coverslip-based technique for evaluating Staphylococcus aureus biofilm formation on human plasma.前细胞感染微生物。2012;2：39。doi: 10.3389/fcimb.2012.00039.eCollection2012。PubMed PMID: 22919630;PubMed Central PMCID：PMC3417647。

Lin LY, Tiemann KM, Li Y, Pinkner JS,沃克JN，Hultgren SJ，Hunstad DA，Wooley KL。Synthetic polymer nanoparticles conjugated with FimH(A) from E. coli pili to emulate the bacterial mode of epithelial internalization.J Am Chem Soc。2012年3月7日；134(9)：3938-41。doi: 10.1021/ja2091917.epub2012Feb 23.PubMed PMID: 22360307;PubMed Central PMCID：PMC3325780。

Chen SL，Hung CS，Pinkner JS，沃克JN，Cusumano CK，Li Z，Bouckaert J，Gordon JI，Hultgren SJ。阳性选择还鉴定出除甘露糖结合外，在尿路感染期间FIMH的体内作用。Proc Natl Acad Sci U S A。2009年12月29日；106（52）：22439-44。doi: 10.1073/pnas.0902179106.epub200912月16日。PubMed PMID: 20018753;PubMed Central PMCID：PMC2794649。

Cegelski L，Pinkner JS，Hammer ND，Cusumano CK，Hung CS，Chorell E，Aberg V，V，沃克JN, Seed PC, Almqvist F, Chapman MR, Hultgren SJ.小分子抑制剂靶向大肠杆菌淀粉样生物发生和生物膜形成。Nat Chem Biol。2009Dec;5(12):913-9。doi：10.1038/nchembio.242。epub2009Oct 25.PubMed PMID：19915538;PubMed Central PMCID: PMC2838449。

Mysorekar IU，Isaacson-Schmid M，沃克JN，Mills JC，Hultgren SJ。Bone morphogenetic protein 4 signaling regulates epithelial renewal in the urinary tract in response to uropathogenic infection.Cell Host Microbe。2009May 8;5（5）:463-75。doi: 10.1016/j.chom.2009.04.005.PubMed PMID: 19454350;PubMed Central PMCID: PMC2696285。

Henderson JP, Crowley JR, Pinkner JS,沃克JN，Tsukayama P，Stamm WE，Hooton TM，Hultgren SJ。Quantitative metabolomics reveals an epigenetic blueprint for iron acquisition in uropathogenic Escherichia coli.PLoS Pathog。2009Feb;5（2）：E1000305。doi：10.1371/journal.ppat.1000305。epub2009Feb 20.PubMed PMID: 19229321;PubMed Central PMCID: PMC2637984。

Rosen DA, Pinkner JS, Jones JM,沃克JN，Clegg S，Hultgren SJ。利用肺炎肺炎尿路感染的细胞内细菌群落途径以及FIMK对1型PILUS表达的影响。Infect Immun。2008Jul;76(7)：3337-45。doi: 10.1128/IAI.00090-08.epub2008Apr 14.PubMed PMID：18411285;PubMed Central PMCID: PMC2446714。

Rosen DA, Pinkner JS,沃克JN，Elam JS，Jones JM，Hultgren SJ。Molecular variations in Klebsiella pneumoniae and Escherichia coli FimH affect function and pathogenesis in the urinary tract.Infect Immun。2008Jul;76(7):3346-56。doi: 10.1128/IAI.00340-08.epub2008May 12.PubMed PMID: 18474655;PubMed Central PMCID：PMC2446687。

Hannan TJ，Mysorekar IU，Chen SL，沃克JN，Jones JM，Pinkner JS，Hultgren SJ，种子PC。LeuX tRNA-dependent and -independent mechanisms of Escherichia coli pathogenesis in acute cystitis.Mol Microbiol。2008年1月；67（1）：116-28。doi: 10.1111/j.1365-2958.2007.06025.x.epub200711月25日。PubMed PMID: 18036139;PubMed Central PMCID: PMC3675907。